Table 1 Primers used for qPCR targeting 5′UTR and near full-length EV PCR amplification.

From: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples

Region

Round

Sense

EV species target

Primer name

Sequence (5′-3′)b

Genome binding positionc

NFGa

RT

—

All

vir21*

ATAAGAATGCGGCCGCTTTTTTTTTTTTTTTTTTTTTTTTT

—

5′UTR/NFG

qPCR/Outer

OF

All

EV1/vir24**

CAAGCACTTCTGTTTCCCCGG

167

5′UTR

qPCR

OR

All

EV4**

CACYGGATGGCCAATCCAA

645

5′UTR

qPCR

—

All

EV probeˆ

[6FAM]TGTGTCGTAACGGGTAACTCTG[BHQ1]

512

5′UTR/NFG

qPCR/Inner

IF

All

EV2/vir26**

TCCTCCGGCCCCTGAATGCG

448

5′UTR

qPCR

IR

All

EV3**

ATTGTCACCATAAGCAGCCA

602

NFG

Outer

OR

A,B,C

vir20ˆ

TTTTTTTTTTTTTTTTTTTTTTTTTCCGCACCGAATGCGGAGAATTTAC

7426

NFG

Inner

IR

A,B,C

vir43ˆ

CCCTACYRCACCGTTRTCTRGTTCGGT

7377

  1. *Primers were adapted from Lindberg J Vir Methods, 1997. **Primers were adapted from Craig J Clin Micro, 2003. ˆPrimers unpublished.
  2. aNFG; near full-length genome.
  3. bFor degenerate primers, R = A or G, Y = C or T.
  4. cGenome binding position in reference to EV genotype CVB5 strain Faulkner, GenBank accession AF114383.1.
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