Figure 2
From: Improving sample classification by harnessing the potential of 1H-NMR signal chemical shifts
Signals can be misaligned in some sample classes. Low pH mediated by the condition studied increases the chemical shift of the signals. The resulting class-dependent signal misalignment can distort the results of the analysis of fingerprint data: features can show significant differences caused by differences in chemical shift (mediated by pH or ionic strength) rather than by differences in metabolite concentration.
