Figure 2 | Scientific Reports

Figure 2

From: Indibulin dampens microtubule dynamics and produces synergistic antiproliferative effect with vinblastine in MCF-7 cells: Implications in cancer chemotherapy

Figure 2

Effects of indibulin on interphase microtubules in MCF-7 cells: (A) Cells were incubated with vehicle or different concentrations of indibulin for 24 h and were then stained with an antibody against α-tubulin (red). DNA stained with Hoechst is shown in blue. Scale bar = 10 µm. (B) MCF-7 cells were treated with vehicle or 150, 450 and 900 nM indibulin (lanes 1–4, respectively) for 48 h. 25 nM vinblastine (lane 5) was used under similar experimental conditions as a control. Polymeric and soluble tubulin fractions were isolated, loaded separately on two different SDS-PAGEs and immunoblotted with the α-tubulin antibody.

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