Figure 2

Reactive oxygen species (ROS) detection. (A) H2O2 accumulation under chilling conditions was visualized by DAB staining in mulberry leaves. (B) \({{\rm{O}}}_{2}^{\bullet -}\) generation was visualized by NBT staining as blue coloration in mulberry leaves. (C) Detection of SOD activity in the mulberry leaves. (D) Analysis of SOD-related gene expression in the three mulberry varieties.