Figure 5 | Scientific Reports

Figure 5

From: The molecular chaperon AKR2A increases the mulberry chilling-tolerant capacity by maintaining SOD activity and unsaturated fatty acids composition

Figure 5

Expression analysis of mAKR2A, mSOD1, mKCS1 and mFADII proteins in mulberry leaves. (A) Total leaf protein extracts were prepared from 20-day-old plants under normal or chilling conditions and analyzed by SDS-PAGE and immunoblotting using anti-AKR2A, anti-SOD1, anti-FADII, anti-KCS1 or anti-Actin antibodies, as indicated. N, normal condition, 25 °C for 14 days; C, chilling condition, 25 °C for 14 days and 2 °C for 2 days. (B) Analysis of SOD1, FADII, KCS1 and AKR2A expression levels. The expression level data from A were analyzed using ImageJ (http://rsb. info. nih. gov/ij/). (C) The proposed mulberry chilling-resistance model. Cold stress induces FADII expression and reduces AKR2A and SOD1 expression. AKR2A positively regulates the expression of SOD1 and FADII. SOD1 can increase the plant chilling resistance by clearing the ROS induced by the chilling stress. FADII improves the plant chilling resistance by increasing the unsaturated fatty acid composition. Arrows denote positive effects, and bars indicate negative roles.

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