Figure 1 | Scientific Reports

Figure 1

From: HA1077 displays synergistic activity with daclatasvir against hepatitis C virus and suppresses the emergence of NS5A resistance-associated substitutions in mice

Figure 1

Inhibition of HCV entry by HA1077. (a) Molecular structure of HA1077. (b) Huh7 cell pre-treated with HA1077 (20 μM) for 24 h were infected with HCV or HCV-infected cells were treated with HA1077. Intracellular HCV RNA loads were determined by RT-qPCR 2 days after viral infection. (c,d) Entry of PKH67 dye-labeled HCV was monitored in HA1077-pretreated Huh7 cells by fluorescent microscopic analysis (c) and quantification of fluorescence intensity (d). Images were representative of at least three independent experiments and were taken 6 h post-infection. (e,f) Effect of ROCK1 or ROCK2 depletion on HCV entry was analyzed as in (c,d) 2 days after treatment with the indicated siRNAs (50 nM). The immunoblots (IB) are representative of two independent experiments. In all panels, data are mean ± s.d. **P < 0.01 by unpaired two-tailed Student’s t-test. n.s., not significant. Scale bar, 50 μm.

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