Figure 2
Haematoxylin & eosin (H & E) stained rat eye histopathology using light microscope at 40X magnification (a) cross section of a normal rat corneal stroma (C) and retina (R) (b) 25 µg/ml capsaicin treated corneal and retinal section showed possible corneal separation and disfiguration from the retina (marked with an arrow) along with corneal vacuolation and disfiguration (marked as**) (c) control corneal stroma with circled areas depicting nuclei of the stromal cells (d) 50 µg/ml capsaicin treated corneal stroma depicting stromal swelling, nuclear aggregation (circled) and vacuolar seperations (indicated by asterisks) (e) 100 µg/ml capsaicin treated corneal section with distinct inflammation in the (1) stratified squamous epithelia containing wing and basal cells, (2) corneal stroma with occasional cracks in it, (3) very indistinct posterior limiting membrane (f) 100 µg/ml capsaicin H & E treated retinal section magnification showing capsaicin induced variations in (1) choroid, (2) retinal pigment epithelium, (3) outer nuclear layer, (4) outer plexiform layer, (5) inner nuclear layer, (6) inner plexiform layer, (7) retinal ganglion cell layer. Observations here include depletion of the ganglion cell layer (7) while the plexiforn layers (4 and 6) remained indistinct. Very little broken choroidal epithelia (1) could be seen which did not form a continous layer.
