Figure 3
Bovine corneal opacity and permeability assay (a) Light microscope observations of H & E stained histopathology sections at 40X of bovine corneas exposed to (a) PBS 7.4 and capsaicin treated (b) 5 mg/ml, (c) 25 mg/ml and (d) 50 mg/ml. As suggested Images suggest certain features such as cytoplasmic breakdown, vacuolar gaps (marked as “v”) and corneal mineralization (marked as “m”) were more prominent in corneas exposed to higher doses of capsaicin. Slight cytoplasmic breakdown can be also observed in 3(b) which is more conspicuous in Fig. 3(c,d), thereby indicating symptoms of predominant inflammation.
