Figure 1
From: The Possible Role of Neurobeachin in Extinction of Contextual Fear Memory
Proteomic analysis identified NBEA as one of the candidate molecules regulated by contextual fear retrieval in the hippocampus. (A) Schematic representation of the experimental protocol. Mice in the control group were fear conditioned with a shock in context A and underwent a retrieval test in context B. Mice in the retrieval group were fear conditioned with a shock in context B and underwent a retrieval test in the same context (context B). (B) Freezing level (%) during 5-min retrieval test. Freezing level in the retrieval group (61.19 ± 5.283, n = 10) was significantly higher than that in the control group (20.48 ± 5.381, n = 10), ***p < 0.0001, t-test, two-tailed. (C) Western blot image of NBEA expression in the control and retrieval group. GAPDH was used for the loading control. (D) Intensity measurement of the Western blot. NBEA expression was significantly higher in the retrieval group (1.541 ± 0.1187, n = 3) than in the control group (0.9913 ± 0.1091, n = 3), *p = 0.0327. NBEA expression was significantly higher in the retrieval group (1.541 ± 0.1187, n = 3) than in the home cage (HC) group (1 ± 0.1077, n = 3), *p = 0.0307, one-way ANOVA, Tukey’s multiple comparisons post hoc test. (E) Schematic representation of the experimental protocol. Mice in the NC (neutral context) retrieval group were handled as the same as the retrieval group with no shock of Day 1. (F) Freezing level in the retrieval group (60.89 ± 4.415, n = 3) was significantly higher than that in the NC (neutral context)-retrieval group (18.31 ± 3.179, n = 3), **p = 0.0014, t-test, two-tailed. (G) Western blot image of NBEA expression in the home cage (HC), NC-retrieval and retrieval groups. GAPDH was used as the loading control. (H) Intensity measurements of the Western blots. NBEA expression was significantly higher in the retrieval group (2.193 ± 0.05042, n = 3) than in the HC group (1 ± 0.01841, n = 3), ****p = 0.0001. NBEA expression was significantly higher in the retrieval group (2.193 ± 0.05042, n = 3) than in the NC-retrieval group (1.084 ± 0.04583, n = 3), ****p = 0.0001. There was no difference between the NC-retrieval group (1.084 ± 0.04583, n = 3) and the HC group in NBEA expression, p = 0.3146, one-way ANOVA, Tukey’s multiple comparisons post hoc test.
