Figure 3
Induction of chronic kidney injury. (A) Kidney weight, creatinine, and BUN; kidney injury score and infiltration of inflammatory cells, wild type mice underwent surgery or intervention and kidneys were harvested 30 days later. Renal sections were stained with Masson’s trichrome (MT) (B), F4/80 (C) and proximal tubules (D). Images illustrate representative sections. The indices for cell infiltration or fibrosis were determined by quantitative morphometry as described in the methods section. Values represent means ± SEM from 30 high power fields of investigated kidneys from 6–10 mice in each group. Data are expressed as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, versus control animal group of the same time point.
