Figure 3 | Scientific Reports

Figure 3

From: Unbiased shRNA screening, using a combination of FACS and high-throughput sequencing, enables identification of novel modifiers of Polycomb silencing

Figure 3

Evaluation and verification of our screening strategy. (A) The 434 final candidate genes (Supplementary Table S3) were analysed using the PANTHER classification system with regard to cellular component enrichment. The top 30 terms ranked according to fold enrichment are shown. (B) Screening results of the representative shRNA constructs for each Polycomb group gene or its related gene are listed2. (C) Scatter plot of 25 of a part of genes listed in (B), with significantly enriched shRNA constructs represented by coloured spots (orange, P < 0.05; red, P < 0.01). PRC2 core components are labelled.

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