Figure 4 | Scientific Reports

Figure 4

From: Unbiased shRNA screening, using a combination of FACS and high-throughput sequencing, enables identification of novel modifiers of Polycomb silencing

Figure 4

Examples of newly identified genes. (A) A graphical summary of a novel function of Mbf1, the Drosophila counterpart of mammalian Edf1. Mbf1 is a known nuclear coactivator promoting an interaction between an activator and a transcriptional preinitiation complex under stress conditions. We recently reported that cytosolic Mbf1 protects E(z) mRNA from Pacman attack under non-stress conditions, thereby ensuring robust Polycomb silencing20. (B and C) HTS analyses of (B) Prdm5 and (C) Setd5. Left panel, Venn diagrams analysing the impact of the differentially expressed genes (DEG) in each knockout (KO) in the ESC modules. DEG, |log2-fc| > 1 and P < 0.01. Statistical analysis of the relationship between the PRC module against either the Myc module or the Core module was done by the Chi-square test. Prdm5, P < 10−134; Setd5, P < 10−98. Right panel, metagene analyses of the histone H3K27me3 level in the indicated genomic region by plotting log2-fold enrichment (log2-fe) against input data. PRC-Module, Kim et al.25; Bivalent Genes, Mikkelsen et al.42.

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