Figure 3

Schematic illustrations of the evaluation of uneven degrees of cracking lines. The total lengths of the outlines in binarized images before (L) and after (L’) Gaussian filtering for a length scale of 27.6 µm (corresponding to 6 pixels), where the distance between neighboring pixels in the original image is 4.6 µm. The bar graph shows the difference in L/L’ for tissues with different disease states. (Mean ± SE, ^*p < 0.05, ^**p < 0.01, head count of the mouse N = 4).