Figure 4

Time profile of the biomass density in the control culture C (●), culture H treated with H₂O₂ (▲), culture H + A treated with H₂O₂ followed by the antioxidant mannitol (■) and culture M treated with menadione (♦). Oxidative stress led to decreased growth and biomass density. Oxidative stress was induced in the bacterial cultures H and M with pulses of H₂O₂ and menadione, respectively, after 5.5 h and 11.5 h. The culture H + A was treated with H₂O₂ after 5.5 h and then with the hydroxyl radical scavenger mannitol after 8.5 h. Values are expressed as mean ± SD, n = 3.