Figure 3 | Scientific Reports

Figure 3

From: Multiplex mutagenesis of four clustered CrRLK1L with CRISPR/Cas9 exposes their growth regulatory roles in response to metal ions

Figure 3

Characterization of the CRISPR/Cas9 edited mutants mds4GG, mds12NN, mds11aZ1 and mds11aZ3. Sequences of mutants with (a) deletions between MDS1 and 4 and (b) small mutations within individual MDS genes. gRNAs are marked by colored boxes and sequences, protospacer adjacent motif (PAM) motifs are indicated in purple. Underlined letters indicate the reading frames. (c) Schematic representation of the mutations. Numbers indicate deleted or inserted bases. (d) Domain structure of the putative truncated and chimeric proteins. Black bars represent deletions.

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