Table 1 Summary of the screening for BASTA sensitive CRISPR/Cas-9 edited T2 plants with deletions between MDS genes and mutations within MDS genes.

From: Multiplex mutagenesis of four clustered CrRLK1L with CRISPR/Cas9 exposes their growth regulatory roles in response to metal ions

Line

# plants tested

BASTA sensitive

Δ mds 1-4

%

Δ mds1-2

Δ mds2-3

Δ mds 3-4

Δ mds1-3

Δ mds2-4

mds1

mds2

mds3

mds4

2

19

7

0

          

3

120

20

5

25.0

1

0

1

5

0

3

6

1

3

4

13

4

1

25.0

2

0

1

0

0

    

8

20

3

0

          

9

65

5

0

      

0

3

1

0

11

108

15

1

6.7

1

0

0

2

0

6

7

6

8

12

119

9

2

22.2

1

1

0

0

1

4

1

0

2

13

100

16

4

25.0

0

0

0

0

0

2

7

2

1

18

15

3

0

          

22

119

24

4

16.7

1

0

1

1

0

2

9

3

8

26

20

3

0

          

Sum

718

109

17

15.6

6

1

3

8

1

17

33

13

22

  1. # plants tested, show the numbers of plants used to examine BASTA sensitivity with the brushing method. % refers to the percentage of BASTA sensitive plants that contain the mds1-4 deletion. The Δ columns display the mutants with smaller deletions between the numbered MDS genes. The columns mds1, mds2, mds3, mds4 correspond to the number of mutant alleles within individual MDS genes.
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