Figure 2
From: Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)
Molecular characterization of jm-iPSCs (J5F1) (a). Chromosomal analysis of jm-iPSCs (J5F1). Scale bar; 10 µm. (b) Live staining of jm-iPSCs (J5F1) with rBC2LCN lectin. Scale bar; 200 µm. (c) Immunofluorescence analyses of pluripotency-associated proteins OCT4, NANOG, LIN28, DPPA4, SOX2, and SALL4. (d) Immunofluorescence analyses of pluripotency-associated cell surface antigens SSEA4, TRA-1-81, E-CADHERIN, and R-10G. Nuclei were counterstained with DAPI. Scale bar; 250 µm. (e) RT-PCR analyses of pluripotency-associated genes. β-ACTIN was examined as an internal control, and water was used as a negative control. Full-length gels are presented in Supplementary Figure S1.
