Figure 2

Differential expression analysis. (A) Heat-map shows the 857 differentially expressed genes clustered by their expression levels in response to M. circinelloides R7B strain infection. Roman numbers and left column colors indicate independent clusters. Color key indicates the log2 fold change values. (B) The graph shows the most specific biological process enriched. (C) The graph shows the most specific molecular function enriched. In (B,C), X-axis indicates the enrichment ratio obtained by dividing the sequences percentage of the test set versus the sequences percentage of the reference set (see methods section). Positive and negative values, respectively represent biological processes and molecular function enriched in up-regulated, and down-regulated genes. AMC, aminoglycan catabolic process; ATA, ATPase activity; BCU, blood coagulation; CBI, calcium ion binding; CRE, cellular response to estrogen stimulus; CTT, carbohydrate transmembrane transport; DRB, defense response to bacterium; EIA, endopeptidase inhibitor activity; HEB, heme binding; HOG, hydrolase activity, hydrolyzing O-glycosyl compounds; INB, iron ion binding; IRE, immune response; LPT, lipid transport; MOM, monocarboxylic acid metabolic process; ORP, oxidation-reduction process; OXI, oxidoreductase activity; PAC, protein activation cascade; PEC, peptidoglycan catabolic process; PRO, proteolysis; RIR, regulation of immune system process; ROC, response to organic cyclic compound; SAR, sarcomere organization; SEP, serine-type endopeptidase activity; SMC, small molecule catabolic process; STT, sodium ion transmembrane transporter activity; TAC, transporter activity; TTA, active transmembrane transporter activity; UTT, urea transmembrane transport; VCM, ventricular cardiac myofibril assembly. Asterisks indicate the statistical significance level, FDR ≤ 0.001 (***), FDR ≤ 0.01 (**) and FDR ≤ 0.05 (*).