Figure 3
From: Hematoxylin and eosin staining of intact tissues via delipidation and ultrasound

Slices from an intact mouse brain stained with iHE, followed by 2D imaging. (A) 3D projection of 20 slices in (B). (B) The coronal plane slices from the C57BL/6 mouse brain after iHE. Objective lens, 4× and 20×; N.A., 0.2 and 0.75. The brain was sliced at the coronal plane for 8 μm, and we selected one slice for every 400 μm from the olfactory bulb to the epencephalon. (D–F) Magnification of (C).