Figure 4

TDZ inhibits LPS-induced IkB degradation in RAW 264.7 macrophages. (A) Immunofluorescence confocal microscopy of IkBα in RAW 264.7 macrophages. RAW 264.7 macrophages were treated with an anti-IkBα antibody to determine the level of IkB (red). Nuclei were counterstained with DAPI (blue) and slides were visualized using confocal microscopy. Merged images of the red and blue fluorescence are shown. Original images ×800 for all panels. The images are representative of three independent preparations. (B) Bar graph showing the mean fluorescent intensities of IkBα quantified from the images shown in A. (C,D) RAW 264.7 macrophages were treated with LPS (250 ng/ml) with or without TDZ (10 μM) for indicated time points and RIPA lysed cells were subjected to western blotting using antibodies specific for IkBα and β-actin. All data are representative of three independent experiments; all are presented as mean ± SD. P Significance was determined using Fisher’s exact test. ^**P < 0.005; ^§§P < 0.001.