Figure 4 | Scientific Reports

Figure 4

From: Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome

Figure 4

Effect of urea supplementation (0, 20, or 40 mg/dl) in maturation medium on the net depletion/appearance of semi-essential amino acids by bovine cumulus-oocyte complex, oocytectomized cumulus cells, or denuded oocytes after 24-h incubation. U0: control group (without urea); U20: 20 mg/dl urea; U40: 40 mg/dl urea. COC: cumulus-oocyte complex; CC: cumulus cells; DO: denuded oocytes; U: the main effect of urea; S: the main effect of specimens (cell type: COCs, DOs, and CCs); U × S: interaction between urea and cell type. Data are presented as least squares means (LSM) ± standard error mean (SEM) indicated by two-way ANOVA over PROC GLM. Different letters (a, b, c, etc.) indicate significant differences between the treatments at P < 0.05. NS: non-significant.

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