Figure 1
Co-localization of PrPSc with the components of machineries involved in retrograde transport from the endosomes to the TGN in ScN2a-3-22L cells. ScN2a-3-22L cells grown on the Chambered Coverglass for 72 h were subjected to IFA. The cells were stained with mAb 132 for PrPSc, antibodies against CHC, Clint1, and Ap1g1 (component molecules of CCVs), antibodies against Snx1, Vps26, Vps29, and Vps35 (component molecules of the retromer complex), and antibodies against Rab9 and Tip47 (component molecules of the Rab9/Tip47 pathway). The cell nuclei were counterstained with DAPI. The leftmost column shows merged images of PrPSc (green), with the component molecules indicated on the left (red), and nuclei (blue). The other three images in the row correspond to high-magnification images of the boxed region for the merged image of PrPSc and nuclei (second left), the merged image of the component molecules and nuclei (second right), and the merged images of PrPSc with the component molecules and nuclei (far right). The arrows indicate representative examples of areas where PrPSc and the corresponding component molecules are co-localized. The co-localization areas were defined as pixels that were positive for both PrPSc signals and signals of the corresponding component molecules. The ratios of PrPSc signals co-localized with the signals of corresponding component molecules relative to the total signals of PrPSc are shown in Supplementary Fig. S1a. Scale bars: 10 µm.
