Figure 2
Co-localization of PrPSc with the components of machineries involved in retrograde transport from the endosomes to the TGN in ScGT1-7-22L cells. ScGT1-7-22L cells grown on the Chambered Coverglass for 96 h were subjected to IFA to monitor PrPSc and the component molecules of CCVs, as well as those of the retromer complex and Rab9/Tip47 pathway. The leftmost column shows lower magnifications of merged images of PrPSc (green), the component molecules (red), and nuclei (blue). Individual and merged high-magnification images of the boxed regions are shown on the right. The arrows indicate representative examples of areas where PrPSc and the corresponding component molecules are co-localized. The co-localization areas were defined as pixels that were positive for both PrPSc signals and signals of the corresponding component molecules. The ratios of PrPSc signals co-localized with the signals of corresponding component molecules relative to the total signals of PrPSc are shown in Supplementary Fig. S1b. Scale bars: 10 µm.
