Figure 5
The effect of the knockdown of Clint1 and Ap1g1 on the amount of PrP in N2a-3 and ScN2a-3-22L cells. N2a-3 and ScN2a-3-22L cells were grown on 6-well plates. SiRNAs against Clint1 and Ap1g1 and negative control siRNA were transfected into the cells by lipofection. Seventy-two hours after the initiation of transfection, the cells were subjected to immunoblot analysis to monitor Clint1, CHC, Ap1g1, PrPC, total PrP, and PrP-res. β-actin was used as a loading control. The immunoblot images of the triplicate samples of N2a-3 and ScN2a-3-22L cells that were transfected with siRNA against Clint1 (siClint1) and negative control siRNA (siNC) are shown in (a). The immunoblot images of triplicated samples of ScN2a-3-22L cells that were transfected with siRNA against Ap1g1 (siAp1g1) and negative control siRNA (siNC) are shown in (b). The cropped blots are shown in the figure, and full-length blots are presented in Supplementary Figs S11 and S12. The graphs at the bottom show the levels of Clint1, Ap1g1, PrPC, total PrP, and PrP-res relative to the average of the control samples transfected with negative control siRNA. Mean and standard deviations (SDs) of triplicate samples are depicted. Asterisks indicate a significant decrease compared to the control (Student’s t-test, p < 0.05).
