Figure 2
Image analysis for studying transendothelial migration in a microfluidic system. (a) Consecutive steps of image processing. (b) (i–iv) Bright field images of the microvessel and reference system respectively, at a particular time point and z-level. (ii–v) Fluorescent images of the cancer cells at a particular time point, inside the microvessel and reference system, respectively. (iii–vi) Segmentation of the projected cancer cell areas (red contours) identified by cell number, in the microvessel and reference system, respectively. The blue lines define the edges of the defined regions. The thickness of the barrier (about 40 μm) is due to the curvature of the collagen interface formed at the gaps between the pillars.
