Figure 1 | Scientific Reports

Figure 1

From: Integrative epigenomic analysis in differentiated human primary bronchial epithelial cells exposed to cigarette smoke

Figure 1

Establishment of air-liquid interface (ALI) to culture human bronchial epithelial (HBE) cells. (A) Expression of Forkhead Box J1 (FOXJ1), Mucin 5AC (MUC5AC) and Regulatory Factor X3 (RFX3) in HBE cells cultured at ALI at different stages measured by RT-PCR. Representative results from multiple repeats (N > 3) were shown here. (B) Pseudostratified architecture and differentiation of HBE cells cultured in ALI by immunofluorescnce staining for beta IV tubulin, MUC5AC, both in red and F-actin in green. Cell nuclei were stained by Hoechst. Side-view images (top panels) were reconstructed from z-stacks, and correspond to a z-slice along the dotted white line in the lower image. Top-view images (bottom panels) are maximum intensity projections over the height indicated by the bracket in the upper image. Scale bars are 20 µm. (C) Epithelial barrier integrity was quantified by TEER (trans-epithelial electrical resistance) measurements. Means ± SD are from 6 wells per time point.

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