Figure 4
Gene expression analysis of HBE cultured at ALI and exposed to sub-chronic cigarette smoke. (A) Principal component analysis (PCA) showing the clustering of all samples in microarray analysis. Samples collected on the same day and condition are circled to help highlight their co-localization on the PCA plot. (B) A heat map showing the GO categories enriched in genes differentially expressed between smoke and air samples in each day. GO categories were selected for display if they were statistically-enriched at a Benjamini-Hochberg corrected p-value < 0.01 and at least 10% of the genes in the gene-set are also annotated to the GO category. Red colors indicate that genes associated with given GO category showed increased expression in smoke-exposed samples compared to air samples; blue colors indicate that genes associated with given GO category showed decreased expression in smoke-exposed samples compared to air samples.
