Figure 5 | Scientific Reports

Figure 5

From: Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips

Figure 5

Limit of detection (LOD) with paper microfluidic RT-LAMP. (a) Representative, raw, green and red channel smartphone images of paper chips with varied ZIKV concentrations from 1 to 1000 copies/µL. (b) Plot of normalized green to red (G/R) intensity against ZIKV concentration, showing the LOD of 1 ZIKV copy/µL using the paper microfluidic RT-LAMP. Average of three different experiments, each using a different paper microfluidic chip, sample, and reagent. Error bars represent standard errors. (c) Standard deviations of green intensities across the detection areas of the paper microfluidic chips, plotted against ZIKV concentration.

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