Figure 6

The effect of sample matrices to conventional RT-LAMP. ZIKV was spiked into tap water (undiluted), human urine (undiluted), and human blood plasma (diluted to 10% using PlasmaLyte), with final concentration of 10 copies/μL. (a) Images of reaction tubes after 15 minutes of reaction. Yellow coloration indicates successful amplification, i.e., all of them are not successful. (b) The gel images after 15 minutes of reaction, again showing either non-specific amplification (tap water) or no amplifications (urine and plasma).