Figure 7
The effect of sample matrices to paper microfluidic RT-LAMP. ZIKV was again spiked into tap water (undiluted), human urine (undiluted), and human blood plasma (diluted to 10% using PlasmaLyte), with final concentration of 10 copies/μL. (a) Representative raw, green and red channel images of paper after 15 minutes of reaction, showing different colorations between positive and NTC samples. (b) The gel images after 15 minutes of reaction show non-successful amplifications, indicating the amplification time was not long enough or the elution from paper was not optimum. (c) Normalized green to red (G/R) intensity values were obtained from smartphone images, indicating successful distinction between positive and NTC samples, thus superiority of this smartphone-based assay. Average of three different experiments, each using a different paper microfluidic chip, sample, and reagent. Error bars represent standard errors.
