Figure 3
From: Characterization of lasR-deficient clinical isolates of Pseudomonas aeruginosa
LasR dependence of OdDHL production. (a) Schematic diagram shows domain structure of LasR and the location of W60* mutation. (b) OdDHL levels assessed by a reporter assay. Extracts of autoinducer from the supernatant of bacterial culture grown for 18 hours were added into the agar plates containing the reporter strains. After incubation at 30 °C for 24 hours, the gray values were measured. The LasR mutants (bj13 and bj14) and the relevant LasR complemented strains (bj13lasR & bj14lasR) are shown for comparison. PAO1, ΔlasR, and the ΔlasR+were used as the controls. The gray values are presented as the mean ± SDs of tests performed in triplicates in three different occasions. ****P<0.0001; P > 0.05, ns (no significance). P values were obtained by one way ANOVA with Bonferroni test.
