Figure 4

SOX stem cell factors mediate activity of PR-LncRNAs. (A) Analysis of the association between expression of PR-LncRNAs and stem cell factors in human glioma samples, showing there is a significant inverse correlation between PR-LncRNAs and SOX members. (B) Correlation between PR-LncRNAs and SOX factors in human glioma samples. Spearman Correlation Rho is −0,546 (**), −0,221 and −0,342 (*) for SOX1, SOX2 and SOX9 with PR-LncRNA1. It is −0,670 (***), −0,151 and −0,285 (*) for SOX1, SOX2 and SOX9 with PR-LncRNA5. Finally numbers are −0,521 (**), −0,171 and −0,230 for SOX1, SOX2 and SOX9 with PR-LncRNA10. (C) Violin plots associated to the expression distribution of the PR-LncRNAs (green circles) and SOX factors (red circles). Values in indicated cells are relative to expression of each transcript in U87-MG cells. The + denote the mean values of the distributions and the black dots are the expression values. (D) Quantitative reverse transcription polymerase chain reaction of SOX1, SOX2 and SOX9 in U87-MG control and transfected with ASOs. Data represents the average of 3 independent experiments. (E) Quantification of the number of P-H3 positive cells in U87-MG cells transfected with indicated ASOs and lentivirally infected with shSOX1, shSOX9 or empty vector (pLKO) (n = 2). (F) Quantification of the number of formed oncospheres in U87 cells transfected with indicated ASOs and lentivirally infected with shSOX1, shSOX9 or empty vector (pLKO) (n = 2). P values were determined by Student’s t test. Asterisks (*^,**) indicate statistical significance (p < 0.05 and p < 0.01).