Figure 3
From: ZNF542P is a pseudogene associated with LDL response to simvastatin treatment
ZNF542P statin response is negatively correlated with intracellular cholesterol ester and plasma LDLC statin responses. Statin-induced changes in ZNF542P expression levels were quantified in 104 European American CAP LCLs after in vitro exposure to 2 μM simvastatin or control buffer. (a) ZNF542P expression changes were tested for correlation with statin induced change in cellular cholesterol ester (CE) quantified in the statin and control treated LCLs from 103 European Americans. Delta cholesterol ester was calculated as the log difference of the statin minus control treated cells. (b) ZNF542P expression levels were quantified by RNA-seq in CAP LCLs from 25 European American LDLC high responders and 25 European American LDLC low responders after 24 hr in vitro incubation with 2 μM simvastatin or control buffer. P-values were calculated using empirical Bayes moderated t-statistics with 5000 permutations to indicate differences in ZNF542P expression changes between high and low responders. For graphical purposes, ZNF542P fold change was estimated as 2^(variance stabilized control-variance stabilized statin) since variance stabilization is roughly a log2 transformation, but the statistics were performed on the variance stabilized delta data after correction for 15 PCs. Individual data points with mean ± s.e.m. are shown.
