Figure 6 | Scientific Reports

Figure 6

From: A singular nitric oxide synthase with a globin domain found in Synechococcus PCC 7335 mobilizes N from arginine to nitrate

Figure 6

Growth and pigment contents in Synechococcus PCC 7335. (a) Synechococcus PCC 7335 was grown in ASN III medium without N (−N) or with the addition of 1 mM arginine (Arg) or 1 mM NO3, and with or without 1 mM L-NAME. Culture growth was measured by OD 600. Inset: growth curve to stationary phase of Synechococcus PCC 7335 growing in ASNIII medium followed by absorbance at 600 and 750 nm. Error bars represent ± SE (n = 3). (b) Chlorophyll (Chl) and phycoerythrin (PE) content in Synechococcus cultures grown 15 days in ASN III medium without N (−N) or with the addition of 1 mM Arg or 1 mM NO3. Photographs of the cultures (top) and quantification of pigments (bottom) are shown. Error bars represent ± SE (n = 3). The asterisk indicates significant difference as compared to −N. (Man-Whitney test, p < 0.05).

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