Figure 7

RT-qPCR analysis of cancer related genes in HepG2 cells treated with 20, 40 and 60 µM of indolin-3-one during 48 hours of exposure. *Indicates statistically difference in relative expression compared to the control (p < 0.05) using GAPDH and HPRT1 as references genes. Statistical evaluation of reference gene and target expression levels were performed using the standalone software REST 2009, with efficiency correction. All expression levels, standard errors, 95% confidence index and p values are described in Table S2.