Figure 6

Immunohistofluorescence stainings. (A) APC staining with HLA-DR antibodies (red) after ex vivo baboon lung co-culture with B. pertussis B1917-GFP (green). (B–D) Post-mortem (five weeks post-inoculation) tissue analysis on B. pertussis challenged (B,C) or naive (D) animals. Immunohistofluorescence staining of frozen bronchial slices with either polyclonal anti-B. pertussis LOS-A mouse antibodies (B,D) or nonspecific mouse IgG3 (C) (green). Nuclei are stained with DAPI (blue).