Figure 5
From: PSD-95-nNOS Coupling Regulates Contextual Fear Extinction in the Dorsal CA3
Mechanisms underlying the role of PSD-95-nNOS in regulating BDNF-TrkB signalling. (A) Design of the experiments for (B,C). (B) Effect of intra-CA3 Tat-nNOS1–133 on fear extinction (n = 12–13). (C) PSD-95-TrkB complex level in the dorsal CA3 after extinction (n = 4–6). (D,E) PSD-95-TrkB complex level in the cultured hippocampal neurons treated by Tat-nNOS1–133 ((D) Left: n = 6–7; Right: n = 6–7) or infected with LV- nNOS1–133-GFP ((E) Left: n = 9; Right: n = 7–9). (F) Effect of intra-CA3 ANA-12 on PSD-95-nNOS and PSD-95-TrkB complex levels in the dorsal CA3 (PSD-95-nNOS: n = 6; PSD-95-TrkB). (G) ERK inhibitor reverses the effect of ZL006 on BDNF expression and ERK phosphorylation (n = 6).
