Figure 2
Yields vary markedly between the A2 subdomain variants. (a) SDS-PAGE of purified sfGFP-A2:LTIB complexes. The protein bands corresponding to sfGFP and LTIB are labeled. Some sfGFP proteolysis was evident during these purifications (*). Each lane is a sample from identical purifications of four independent expressions for each construct. The purification strategy yields relatively pure and fully assembled complexes. Densitometry of the expressed complexes is compiled qualitatively in (b). Typical yields are reported from peak integrals for the purifications of each of the complexes. Toxicity for some complexes was evident through diminishing OD600 after induction, autolysed cell pellets after standard expressions, and substantial quantities of B subunit complexes in the media supernatant of pelleted cells.
