Figure 1

miR-125 is downregulated in pilocytic astrocytoma (PA) and other low grade gliomas/glioneuromal tumor compared with non-neoplastic brain. MicroRNA expression data obtained through Agilent arrays demonstrated lower miR-125 expression levels in PA (n = 43) compared to non-neoplastic brain, particularly cerebral cortex (n = 3) (cbll = cerebellum n = 2). Each data point (heatmap square) represents an individual sample. (A) microRNA expression using a nanostring platform demonstrated miR-125 underexpression particularly in pleomorphic xanthoastrocytoma (PXA) (n = 7), ganglioglioma (GG) (n = 6) and rosette forming glioneuronal tumor (RFGT) (n = 2). Each point represent an individual sample. Only these 3 subgroups that demonstrated significant differences between tumor and normal brain are illustrated. Normalized counts were obtained using nSolver software (NanoString) on Raw data filtered for minimum expression threshold, and log2 transformed before analysis as previously reported¹³ (B).