Figure 5

Overexpression of miR-125b decreases cell growth. (A) qRT-PCR of miR-125b-5p expression in pediatric cell lines. (B) Overexpression of miR-125b in Res186, Res259 and BT66 (BRAF fusion+) cells. All data were normalized with miR-control infected cells. (**p < 0.01 compare to miR-control). (C) Cell growth curve of Res186, Res259 and BT66 cells infected with miR-control and miR-125b. (*p < 0.05 and **p < 0.01 compare to miR-control). (D) Quantification of BrdU incorporation is at left and BT66 analyzed data of flow cytometer is at right (*p < 0.05 compare to miR-control). Results represent the average of at least three biological replicates and data were analyzed with a two-tailed Student’s t-test.