Figure 2
From: AMPK contributes to autophagosome maturation and lysosomal fusion
AMPK α1 knockout inhibits autophagy initiation upon starvation. (A) Control cells and AMPK α1 knockout cells were transfected with a plasmid encoding mRFP-GFP-LC3, and cells were either mock-treated or starved in HBSS medium for the indicated time (1 h or 2 h). Bars: 10 μm. (B) The numbers of LC3 puncta in control cells and AMPK α1 knockout cells were counted (n = 20). Control cells vs. AMPK knockout cells, *P < 0.05, **P < 0.0005. (C) AMPK α1 knockout represses starvation-induced autophagy. Control cells and AMPK α1 knockout cells were incubated in HBSS medium, and the cell lysates were probed with anti-LC3 and anti-p62 antibodies. (D) The LC3-II, LC3-I and p62 bands were quantified, and the relative expression levels of LC3-II/LC3-I and p62 are shown in the graph. 0 h vs. 1 h or 2 h, *P < 0.05.
