Figure 3
From: AMPK contributes to autophagosome maturation and lysosomal fusion
AMPK α1 knockout represses autophagic flux. (A) Control cells and AMPK α1 knockout cells were transfected with a plasmid encoding mRFP-GFP-LC3, and cells were either mock-treated (vehicle alone) or treated with compound C (cC, 10 μM) for 18 h. Bars: 10 μm. (B) Ratio quantification of autophagosomal LC3 puncta to autolysosomal LC3 puncta (n = 10). Control cells vs. knockout cells, *P < 0.0001. (C,D) HEK293T cells and AMPK α1 knockout cells were treated with compound C (10 μM) for 18 h, and the cell lysates were probed with anti-LC3 and anti-p62 antibodies. The p62 bands were quantified, and the relative expression levels are shown in the graph. Control cells vs. AMPK α1 knockout cells, *P < 0.05, **P < 0.005.
