Figure 5
From: AMPK contributes to autophagosome maturation and lysosomal fusion
AMPK α1 knockout deregulates AMPK-related signaling. (A) AMPK α1 knockout decreases the phosphorylation level of acetyl-CoA carboxylase (ACC). Control cells and AMPK α1 knockout cells were either mock-treated (vehicle alone) or treated with compound C (cC, 10 μM) for 18 h, and the equal amount of cell lysates were probed with the indicated antibodies. (B) Proliferation of AMPK α1 KO cell lines after compound C treatment. HEK293T control and knockout clones were seeded into 24-well plates and incubated with the indicated concentrations of compound C. MTT assays were performed in triplicate, and the graph shows the average and standard deviation (SD). (C) Cell viability of AMPK α1 KO cell lines were measured with Trypan blue assay after compound C treatment. Control cells vs. knockout cells, NS: not significant.
