Figure 6
From: AMPK contributes to autophagosome maturation and lysosomal fusion
Expression of AMPK α1 recues the AMPK α1 knockout phenotype. (A) AMPK α1 knockout cells were transfected with mRFP-GRP-LC3 in the presence or absence of the AMPK plasmid. Twenty-four h after transfection, cells were treated with compound C for 18 h. Bars: 10 μm. (B) Ratio quantification of autophagosomal LC3 puncta to autolysosomal LC3 puncta (n = 10). Vector vs AMPK α1 plasmid, *P < 0.0001. (C) AMPK α1 knockout cells were transfected with the plasmid encoding AMPK α1. Twenty-four hour after transfection, cells were treated with compound C for 18 h. Equal amounts of cell lysates were probed with the indicated antibodies.
