Figure 8
From: AMPK contributes to autophagosome maturation and lysosomal fusion
Expression of AMPK α1 recovers trehalose induced autophagic flux. (A,B) AMPK α1 knockout cells were transfected with mRFP-GFP-LC3 in the presence or absence of the AMPK plasmid. Twenty-four hour after, cells were treated with trehalose for 6 h. Ratio quantification of autophagosomal LC3 (GFP+/RFP+) puncta to autolysosomal LC3 puncta (GFP−/RFP+) Bars: 10 μm. (n = 15, vector vs AMPK plasmid, *P < 0.0001). (C) AMPK α1 knockout cells were transfected with AMPK plasmid. Twenty-four hour after transfection, cells were treated with trehalose for 6 h. Cell lysates were probed with the indicated antibody.
