Figure 1 | Scientific Reports

Figure 1

From: Unraveling local tissue changes within severely injured skeletal muscles in response to MSC-based intervention using MALDI Imaging mass spectrometry

Figure 1

Discrimination pathophysiological region within injured skeletal muscles of MSC-TX and control group. (a) H&E staining of traumatized soleus muscles from the MSC-TX (left) and control group (right). Primary trauma region are indicated as tm and the trauma adjacent area as tam. (b) Discrimination of tam and tm region by alpha skeletal muscle actin (Acts). The m/z value 976 shows significantly lower intensities (p < 0.05) in the tm regions compared to the tam regions of both group. (c) Spatial segmentation analysis with two segments results in a clear discrimination of tam tissue area (blue region) from the tm (red). (d). A third segment (green region) could be determined exclusively in the tam region of MSC-TX muscle tissue. (e) Intensity distribution of the first principal component PC1 for the considered tissue sections, which clearly discriminates MSC-TX muscles from corresponding regions of the control. Principal component PC2 clearly discriminates between tm and tam region in both groups. (f). Probabilistic latent semantic analysis discriminates tam region of MSC-TX from the corresponding region of the control muscles. First component C1 shows a higher intensity in tam region of control muscles. In contrast, the second component C2 shows an increased intensity exclusive in the tam region of MSC-TX muscles.

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