Figure 7
HL-1 cells incubated with TTR-L55P: analysis of the 3050-2800 cm−1 CHn stretching range. Left panel: second derivatives of the FTIR absorption spectra of HL-1 cells untreated, incubated for 30 min or 24 h with TTR-L55P in the natively folded (top spectra), oligomeric-like (middle spectra) or fibrillar-like (bottom spectra) conformation. Second derivatives are shown after spectra normalization at the CH3 band at 2959 cm−1. Representative spectra are shown, computed as average of the three most central spectra within the space formed by the first three PCA-LDA scores. Right panel: differences of the average (across spectra) linear mixed model response variable (η, see Methods for details) between TTR-L55P-treated (pH7, OL, FB at 30 min and 24 h) and control (NT) cells. Error bars show the 95% confidence interval. Stars above the vertical bar indicate the Dunnet’s adjusted two-sided P-values from two-sample Student t-test. P-value: ** < 0.01.
