Figure 4

Variable location of eGFP transfected cells. GFP expressing cells were also found in suprabasal position in addition to the superficial position shown in Fig. 3b–g. (a) Representative result of suprabasal GFP⁺ epidermal cells 24 h after biolistic transfection, (b) in higher magnification, both showing an overlay of GFP and DAPI signals with bright field (BF) to visualize gold microparticles. (c) Same magnification than in (b) without depicting microparticles. Results from one representative donor (of five tested for GFP transfection) Scale bar 50 µm.