Figure 5
Functionality control of IVT mRNA using biolistic application in ex vivo human skin. (a) Quality control of mRNA loaded bullets. Samples were analysed in a blinded fashion and thereby the nomenclature is resulting in a mixed order on the gel. Left lane always indicates mRNA before loading onto bullets, right lane shows the mRNA after extracting from loaded bullets. Outer lanes depict size markers. (b) Human whole skin explants were transfected using gene gun technology with native IFN-α mRNA or five different mRNA variants (for details see Table 1) compared to untransfected explants (CTRL) and subsequently Hematoxylin/Eosin stainings (HE) on paraffin sections were performed. A set of seven representative areas per sample showing the gold particles after biolistic transfection highlighted by yellow circles indicating targeting of mostly epidermis but also dermis. Scale bar represents 50 µm.
