Figure 6
IVT Interferon mRNA variant -based Interferon protein expression in skin explant tissue. Human whole skin explants were transfected using gene gun technology with native IFN-α mRNA or five different IFN-α mRNA variants (IFN-α var 1–5, for details see Table 1) compared to eGFP reference transfections and un-transfected control explants (CTRL). IFN-α content in explants homogenized 24 h after treatment is shown as pg protein produced per mg skin tissue homogenate. (N = 5; Dunnet’s multiple comparison for the individual variant IFN-α vs. native IFN-α expression; significant differences are indicated ** vs. ****). Data depicted at logarithmic scale.
